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bio rad cfx 96  (Bio-Rad)


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    Bio-Rad bio rad cfx 96
    Bio Rad Cfx 96, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 28823 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+cfx+96+real+time+pcr+system/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm42777485-117-1-1
    Average 96 stars, based on 28823 article reviews
    bio rad cfx 96 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Formoterol promotes mitochondrial biogenesis, improves liver regeneration, and suppresses liver injury and inflammation after liver resection in mice with endotoxemia
    Article Snippet: .. Real-time PCR was performed using iQTM SYBR Green Supermix and a Bio-Rad CFX 96 Real Time PCR System as described [ 35 ]. ..

    Article Title: Mesenchymal stem cells offer a drug-tolerant and immune-privileged niche to Mycobacterium tuberculosis
    Article Snippet: Total RNA from ADSCs was isolated using mdi RNA isolation kit. cDNA was synthesized from 500 ng of total RNA by reverse transcriptase PCR using Bio-RAD iScript cDNA synthesis kit according to the manufacturer’s protocol. .. The cDNA samples were run in triplicate using β-tubulin and actin as normalizing control, respectively, using SYBR green dye for real time fluorescence acquisition on the Bio-Rad CFX 96 Real time PCR system. .. Primers were custom synthesized from Sigma Aldrich Chemicals Ltd. Primers used: ABCC1 (F:CGAGAACCAGAAGGCCTATTAC, R:ACAGGGCAGCAAACAGAA) ABCG2 (F:CTTCGGCTTGCAACAACTATG,R:CCAGACACACCACGGATAAA), Tubulin (F:TTGGCCAGATCTTTAGACCAGACAAC,R: CCGTACCACATCCAGGACAGAATC), Actin (F: ACCTTCTACAATGAGCTGCG, R: CCTGGATAGCAACGTACATGG).

    Article Title: Characteristics of Vibrio vulnificus isolates from clinical and environmental sources.
    Article Snippet: The mastermix was comprised of 12.5 μL Sso Fast Evagreen® Supermix (Bio-Rad Laboratories, Inc., Hercules, CA), 0.3 μm of each primer, and water to volume for 25 μL reactions using 5 μl template DNA. .. Amplification and product detection was performed on a Bio-Rad CFX 96 Real Time PCR System under the following conditions: a 3 min initial denaturation at 94 ◦C, followed by 40 cycles of 94 ◦C for 45 s, 55 ◦C for 45 s, and 72 ◦C for 45 s. Products were visualized in real time using the SYBR green channel and evaluated post-hoc using melt curve analysis. ..

    Article Title: LP340, a novel histone deacetylase inhibitor, decreases liver injury and fibrosis in mice: role of oxidative stress and microRNA-23a
    Article Snippet: .. Real-time PCR was performed using iQTM SYBR Green Supermix ( ) and a Bio-Rad CFX 96 Real time PCR System with incubations at 55°C for 5 min and 95°C for 5 min, followed by 39 cycles at 95°C for 10 s and 60°C for 1 min. ..

    Article Title: Differential effects of alkyl gallates on quorum sensing in Pseudomonas aeruginosa
    Article Snippet: .. For the determination of QS gene expression, total RNA was extracted using TRIzol reagent (Invitrogen), followed by synthesis of cDNA for RT-qPCR detection of the expression of target genes and measurement by RT-qPCR using a Bio-Rad CFX-96 real− time PCR system (Bio-Rad, Hercules, CA, USA) with the primers listed in Table (Supporting Information). mRNA expression was normalized to the expression of the endogenous rpoD gene. ..

    Article Title: Investigating the Antibiotic Resistance Genes and Mobile Genetic Elements in Water Systems impacted with Anthropogenic Pollutants.
    Article Snippet: A wide range of pollutants, including heavy metals, endocrine-disrupting chemicals (EDCs), residual pesticides, and pharmaceuticals, are present in various water systems, many of which strongly drive the proliferation and dissemination of antimicrobial resistance genes (ARGs), heightening the antimicrobial resistance (AMR) crisis and creating a critical challenge for environmental and health management worldwide.. This study addresses the impact of anthropogenic pollutants on AMR through an extensive analysis of ARGs and mobile genetic elements (MGEs) in urban wastewater, source water, and drinking water supplies in India.. Results indicated that blaTEM and blaCTXM-32 were the dominant ARGs across all water systems, underscoring the prevalence and dominance of resistance against β-lactam antibiotics.

    Article Title: Transcriptome analysis of Actinidia chinensis in response to Botryosphaeria dothidea infection
    Article Snippet: The cDNA was synthesized from 4 μL of total RNA using GoScriptTM Reverse Transcription System (Promega, Madison, USA) in 20 μL of reaction mixture. .. RT-qPCR was performed using GoTaq ® qPCR master mix (Promega, Madison, USA) on a Bio-Rad CFX 96 real time PCR system (Bio-Rad, Hercules, CA, USA). ..

    SYBR Green Assay:

    Article Title: Formoterol promotes mitochondrial biogenesis, improves liver regeneration, and suppresses liver injury and inflammation after liver resection in mice with endotoxemia
    Article Snippet: .. Real-time PCR was performed using iQTM SYBR Green Supermix and a Bio-Rad CFX 96 Real Time PCR System as described [ 35 ]. ..

    Article Title: Mesenchymal stem cells offer a drug-tolerant and immune-privileged niche to Mycobacterium tuberculosis
    Article Snippet: Total RNA from ADSCs was isolated using mdi RNA isolation kit. cDNA was synthesized from 500 ng of total RNA by reverse transcriptase PCR using Bio-RAD iScript cDNA synthesis kit according to the manufacturer’s protocol. .. The cDNA samples were run in triplicate using β-tubulin and actin as normalizing control, respectively, using SYBR green dye for real time fluorescence acquisition on the Bio-Rad CFX 96 Real time PCR system. .. Primers were custom synthesized from Sigma Aldrich Chemicals Ltd. Primers used: ABCC1 (F:CGAGAACCAGAAGGCCTATTAC, R:ACAGGGCAGCAAACAGAA) ABCG2 (F:CTTCGGCTTGCAACAACTATG,R:CCAGACACACCACGGATAAA), Tubulin (F:TTGGCCAGATCTTTAGACCAGACAAC,R: CCGTACCACATCCAGGACAGAATC), Actin (F: ACCTTCTACAATGAGCTGCG, R: CCTGGATAGCAACGTACATGG).

    Article Title: Characteristics of Vibrio vulnificus isolates from clinical and environmental sources.
    Article Snippet: The mastermix was comprised of 12.5 μL Sso Fast Evagreen® Supermix (Bio-Rad Laboratories, Inc., Hercules, CA), 0.3 μm of each primer, and water to volume for 25 μL reactions using 5 μl template DNA. .. Amplification and product detection was performed on a Bio-Rad CFX 96 Real Time PCR System under the following conditions: a 3 min initial denaturation at 94 ◦C, followed by 40 cycles of 94 ◦C for 45 s, 55 ◦C for 45 s, and 72 ◦C for 45 s. Products were visualized in real time using the SYBR green channel and evaluated post-hoc using melt curve analysis. ..

    Article Title: LP340, a novel histone deacetylase inhibitor, decreases liver injury and fibrosis in mice: role of oxidative stress and microRNA-23a
    Article Snippet: .. Real-time PCR was performed using iQTM SYBR Green Supermix ( ) and a Bio-Rad CFX 96 Real time PCR System with incubations at 55°C for 5 min and 95°C for 5 min, followed by 39 cycles at 95°C for 10 s and 60°C for 1 min. ..

    Control:

    Article Title: Mesenchymal stem cells offer a drug-tolerant and immune-privileged niche to Mycobacterium tuberculosis
    Article Snippet: Total RNA from ADSCs was isolated using mdi RNA isolation kit. cDNA was synthesized from 500 ng of total RNA by reverse transcriptase PCR using Bio-RAD iScript cDNA synthesis kit according to the manufacturer’s protocol. .. The cDNA samples were run in triplicate using β-tubulin and actin as normalizing control, respectively, using SYBR green dye for real time fluorescence acquisition on the Bio-Rad CFX 96 Real time PCR system. .. Primers were custom synthesized from Sigma Aldrich Chemicals Ltd. Primers used: ABCC1 (F:CGAGAACCAGAAGGCCTATTAC, R:ACAGGGCAGCAAACAGAA) ABCG2 (F:CTTCGGCTTGCAACAACTATG,R:CCAGACACACCACGGATAAA), Tubulin (F:TTGGCCAGATCTTTAGACCAGACAAC,R: CCGTACCACATCCAGGACAGAATC), Actin (F: ACCTTCTACAATGAGCTGCG, R: CCTGGATAGCAACGTACATGG).

    Fluorescence:

    Article Title: Mesenchymal stem cells offer a drug-tolerant and immune-privileged niche to Mycobacterium tuberculosis
    Article Snippet: Total RNA from ADSCs was isolated using mdi RNA isolation kit. cDNA was synthesized from 500 ng of total RNA by reverse transcriptase PCR using Bio-RAD iScript cDNA synthesis kit according to the manufacturer’s protocol. .. The cDNA samples were run in triplicate using β-tubulin and actin as normalizing control, respectively, using SYBR green dye for real time fluorescence acquisition on the Bio-Rad CFX 96 Real time PCR system. .. Primers were custom synthesized from Sigma Aldrich Chemicals Ltd. Primers used: ABCC1 (F:CGAGAACCAGAAGGCCTATTAC, R:ACAGGGCAGCAAACAGAA) ABCG2 (F:CTTCGGCTTGCAACAACTATG,R:CCAGACACACCACGGATAAA), Tubulin (F:TTGGCCAGATCTTTAGACCAGACAAC,R: CCGTACCACATCCAGGACAGAATC), Actin (F: ACCTTCTACAATGAGCTGCG, R: CCTGGATAGCAACGTACATGG).

    Amplification:

    Article Title: Characteristics of Vibrio vulnificus isolates from clinical and environmental sources.
    Article Snippet: The mastermix was comprised of 12.5 μL Sso Fast Evagreen® Supermix (Bio-Rad Laboratories, Inc., Hercules, CA), 0.3 μm of each primer, and water to volume for 25 μL reactions using 5 μl template DNA. .. Amplification and product detection was performed on a Bio-Rad CFX 96 Real Time PCR System under the following conditions: a 3 min initial denaturation at 94 ◦C, followed by 40 cycles of 94 ◦C for 45 s, 55 ◦C for 45 s, and 72 ◦C for 45 s. Products were visualized in real time using the SYBR green channel and evaluated post-hoc using melt curve analysis. ..

    Gene Expression:

    Article Title: Differential effects of alkyl gallates on quorum sensing in Pseudomonas aeruginosa
    Article Snippet: .. For the determination of QS gene expression, total RNA was extracted using TRIzol reagent (Invitrogen), followed by synthesis of cDNA for RT-qPCR detection of the expression of target genes and measurement by RT-qPCR using a Bio-Rad CFX-96 real− time PCR system (Bio-Rad, Hercules, CA, USA) with the primers listed in Table (Supporting Information). mRNA expression was normalized to the expression of the endogenous rpoD gene. ..

    Quantitative RT-PCR:

    Article Title: Differential effects of alkyl gallates on quorum sensing in Pseudomonas aeruginosa
    Article Snippet: .. For the determination of QS gene expression, total RNA was extracted using TRIzol reagent (Invitrogen), followed by synthesis of cDNA for RT-qPCR detection of the expression of target genes and measurement by RT-qPCR using a Bio-Rad CFX-96 real− time PCR system (Bio-Rad, Hercules, CA, USA) with the primers listed in Table (Supporting Information). mRNA expression was normalized to the expression of the endogenous rpoD gene. ..

    Article Title: Transcriptome analysis of Actinidia chinensis in response to Botryosphaeria dothidea infection
    Article Snippet: The cDNA was synthesized from 4 μL of total RNA using GoScriptTM Reverse Transcription System (Promega, Madison, USA) in 20 μL of reaction mixture. .. RT-qPCR was performed using GoTaq ® qPCR master mix (Promega, Madison, USA) on a Bio-Rad CFX 96 real time PCR system (Bio-Rad, Hercules, CA, USA). ..

    Expressing:

    Article Title: Differential effects of alkyl gallates on quorum sensing in Pseudomonas aeruginosa
    Article Snippet: .. For the determination of QS gene expression, total RNA was extracted using TRIzol reagent (Invitrogen), followed by synthesis of cDNA for RT-qPCR detection of the expression of target genes and measurement by RT-qPCR using a Bio-Rad CFX-96 real− time PCR system (Bio-Rad, Hercules, CA, USA) with the primers listed in Table (Supporting Information). mRNA expression was normalized to the expression of the endogenous rpoD gene. ..

    other:

    Article Title: Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors in Matricaria recutita L.
    Article Snippet: The synthesized cDNA was amplified by qPCR using a TaKaRa SYBR Green qPCR mix and a Bio-Rad CFX 96TM real-time PCR system (Bio-Rad, Hercules, CA, USA).



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